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project-m4

Selects 100 oyster (Magallana / Crassostrea) sequencing experiments from an NCBI SRA search export and downloads them.

Task Code Output
01 Select 100 experiments from data/sra_result.csv code/01-select-experiments.py output/01/
02 Download the selected experiments from NCBI SRA code/02-download-sra-data.py output/02/ (data not tracked in git)

Task descriptions are in Tasks.txt; repository conventions are in Instructions.txt.

Setup

pip install -r requirements.txt            # pandas
conda install -c bioconda sra-tools pigz   # needed for Task 02 only

Running

Scripts can be run from any directory.

python3 code/01-select-experiments.py
python3 code/02-download-sra-data.py --threads 8

Task 02 downloads about 525 GB of SRA data and needs substantially more free space while converting to FASTQ. It can be re-run to resume.

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